| Authors |
Rabadanova Aminat Ibragimovna, Candidate of biological sciences, associate professor, sub-department of zoology and physiology, Dagestan State University (43A, M. Gadzhiyeva street, Makhachkala, Russia), E-mail: phisiodgu@mail.ru
Salatgereeva El'vira Gereykhanovna, Master degree student, Dagestan State University (43A, M. Gadzhiyeva street, Makhachkala, Russia), E-mail: ashty06@mail.ru
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| Abstract |
Background. To assess the physiological state of the body, it is important to determine the parameters of the blood, which play a significant role in its adaptation to living conditions, by maintaining homeostasis. Proper understanding of hematopoiesis, composition and properties of blood allows to recognize normal and pathological processes in the body. In the published literature there is not enough information about the parameters of the blood of reptiles of Dagestan, many species of which live in different habitats. The aim of this work was to study the quantitative and qualitative indicators of erythrocytes of two species of lizards (striped lizard, Lacerta strigata (Eichwald, 1831) and the Giant glass lizard, Pseudopus adopus (Pallas, 1775)) living in the lowlands and foothills.
Materials and methods. Lizards were caught in the territories of the Primorsky and Tersk-Sulak lowlands and in the foothills. Qualitative (acid resistance) and quantitative (total number, morphometric parameters) indicators of erythrocytes were determined in reptile blood. The obtained data were subjected to variational statistical processing.
Results. In the blood of striped lizard and giant glass lizard, that living in the lower foothills, the content of blood components involved in the transport of oxygen, does not detect interspecies differences. Adaptation to hypoxia, as expected, is manifested in an increase in the average hemoglobin content in red blood cells in both species of lizards. Interspecific differences can be traced when comparing this parameter in the blood of lizards living on the lowlands. In terms of lowland Dagestan, the content of hemoglobin in erythrocytes is higher in individuals of the striped lizards that live on the coastal lowlands, while the giant glass lizard this parameter is more pronounced in individuals caught North of the Terek-Sulak lowlands. By cytomorphological parameters of erythrocytes there is little interspecific differences, manifested in large amounts of red blood cells P. apodus compared with L. strigata. At the same time, the size of red blood cells in individuals of both species living in the territory of the Tersk-Sulak lowland was larger. Comparison of acid erythrograms of lizards showed interspecific differences, expressed in greater acid resistance of red blood cells of giant glass lizard. This is indicated by a longer duration of hemolysis, a lower percentage of hemolized erythrocytes at the peak, as well as a change in the ratio of low- and high-resistant erythrocytes towards the predominance of the latter. The study of the dependence of the stability of erythrocytes on habitats showed that the erythrocytes of both species of lizards caught in the territory of the Tersk-Sulak lowland are more resistant compared to the erythrocytes of the same lizards living in the Coastal lowland and foothills. This is indicated by the right shift of erythrograms, a decrease in the number of hemolysed erythrocytes at the peak, as well as a decrease in the number of low-resistant erythrocytes. Thus, it can be assumed that in individuals living in the foothills, adaptation to changing landscapes with changing microclimatic conditions is accompanied by an increase in the average hemoglobin content in red blood cells, while the temperature factor in the lowland causes a change in the cytomorphology of red blood cells.
Conclusions. The content of red blood cells and hemoglobin in the blood of lizards depends on both species and habitat. Erythrocytes of both species of lizards caught in the territory of the Tersk-Sulak lowland have large values of surface area, volume and thickness, and also exhibit greater acid resistance, compared with erythrocytes of individuals from the Coastal lowland, located to the South.
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